Tag

Part:BBa_K112507:Design

Designed by: Sherine Cheung   Group: iGEM08_UC_Berkeley   (2008-10-22)


< HA ! in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

Construction of {<HA!} basic part K112507

Wobble PCR of cc001/cc011                  ( 54 bp, EcoRI/BamHI)
Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)
Product is pBca1256-K112507
--------------------------------------------
cc001             Forward Biobricking of {<HA>} 
CgATAgaattcATGagatcttacccatacgacgtcccagac

cc011             Reverse biobricking of {<HA!}
ccagtggatccttacccagcgtagtctgggacgtcgtatgggtaag


References